Yes.
Each workspace acts as a totally independent version of HotHawk. Anything you do in one workspace does not copy over to another workspace.
Every workspace you add will need it's own independent Mailboxes (read more) it's own team members and have it's own sales opportunity board.
When you invite different team members and different clients to a workspace, they cannot see any other workspace or account data, other than the individual workspace(s) you have invited them into.